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  E-cigarettes can damage DNA, ACS reports

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Silvia Balbo, Ph.D and Romel Dator, Ph.D have pushed scientific findings which identify three compounds contained in e-cigarettes which can be damaging to traces of DNA examined in the human lung; known as an adduct, this damage (similar to the effects of acrolein exposure) is typically initiated by the inhalation of toxic chemicals. Dator and Balbo's collective study finds that formaldehyde, acrolein and methylglyoxal levels are significantly increased in the sampled saliva of those who smoke e-cigarettes, as compared to those who don't – these chemicals leading to the aforementioned conditions in deoxyribonucleic acid.

256th congregation of the American Chemical Society, on the characterisation of the effects of electronic cigarette exposure in humans

Here, we have integrated these MS-based methods to characterize electronic cigarette exposure in humans, with the goal of identifying reactive carbonyls generated during vaping and the corresponding DNA adducts formed in the oral cavity. Human saliva and oral cell samples from e-cigarette users (n=10) and non-user controls (n=10) were obtained to screen reactive carbonyls and DNA adducts. Reactive carbonyls in saliva were derivatized with 2,4-dinitrophenylhydrazine to form hydrazones and analyzed by the NL screening method. Likewise, DNA from oral cells were isolated, hydrolyzed to nucleosides, and analyzed by both targeted and non-targeted DNA adductomics approaches. Using the NL loss screening strategy, increased levels of acrolein, methylglyoxal, and formaldehyde were observed after vaping, while the levels of acetaldehyde and glyoxal vary within subjects possibly due to variations in composition of the e-liquid used. This information was then used to develop targeted and non-targeted DNA adductomics approaches to monitor the corresponding DNA adducts in oral cells of e-cigarette users. Our results showed increased levels of acrolein-derived DNA adducts, in particular, gamma-OH-Acro-dG in e-cigarette users compared to non-users. We are currently investigating other DNA adducts that might be relevant to e-cigarette exposure using non-targeted DNA adductomics approaches and expanding our analysis to a larger sample size.

Local governments have increased in the frequency of response to e-cigarette usage by passing laws to cover electronic cigarettes under pre-existing regulations which do not allow their usage on public property.

As of current, further research on the subject is to-be needed; but one may deduce from the studies presented that e-cigarette usage should be avoided, due to dangers which may not be known.

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